Doe urine is the urine of a female deer, often used as a scent attractant during hunting. It is commonly employed by hunters to attract male deer (bucks) by mimicking the presence of a female in estrus or simply signaling the presence of a doe in the area. This scent is particularly effective during the rutting season, when bucks are more actively seeking mates and are highly responsive to the smell of a doe's urine.

Doe urine is typically collected and bottled for use as a hunting tool, either in its natural form or as part of a scent mixture. Hunters often use it in various ways, such as applying it to scrapes, dribbled on the ground, or using it with scent trails to draw deer closer to their position. Some hunters also use it with scent dispersers or scent wicks to carry the aroma through the air and into the hunting area, increasing the chances of attracting a buck or other deer.

The use of doe urine can create an environment where the deer feel comfortable, as it may suggest to them that there is an active, non-threatening female in the area. It can also be used strategically to mask a hunter’s scent, making it harder for deer to detect human presence. While effective, the use of doe urine should be done with care, as improper application or overuse can reduce its effectiveness and potentially make deer wary if they sense the scent is unnatural.